two hybrid y2h bait vector pas2 1 Search Results


pas2-1 vector  (Becton Dickinson)
90
Becton Dickinson pas2-1 vector
HuR interacts with S-HDAg in a yeast two-hybrid assay . ( A ) Schematic representation of HuR and the AD fusion protein expressed in yeast positive clone (AD/HuR). The protein AD/HuR includes a truncated form of HuR, devoid of the first N-terminal 12 aa. GAL-AD represents the activation domain of the GAL4 transcription factor and RRMs represent the RNA recognition motifs in the HuR protein. ( B ) AH109 yeast cells were (co)transformed with the indicated plasmids. Interactions were tested by growth selection on dropout media lacking the amino acids Leu or Trp (SD/-Leu or SD/-Trp) to select for pGADT7/Rec or <t>pAS2-1</t> plasmid constructs, respectively. Activation of reporter genes was tested on plates lacking Leu, Trp, Hist and Ade, and containing X-α-Gal (SD/-Trp/-Leu/-Hist/-Ade/X-a-Gal).
Pas2 1 Vector, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two+hybrid+y2h+bait+vector+pas2+1/pmc03109592-40-13-15?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
pas2-1 vector - by Bioz Stars, 2026-08
90/100 stars
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gal4 dna activation domain vector pact2 (pas2-1-tctp  (Becton Dickinson)
90
Becton Dickinson gal4 dna activation domain vector pact2 (pas2-1-tctp
HuR interacts with S-HDAg in a yeast two-hybrid assay . ( A ) Schematic representation of HuR and the AD fusion protein expressed in yeast positive clone (AD/HuR). The protein AD/HuR includes a truncated form of HuR, devoid of the first N-terminal 12 aa. GAL-AD represents the activation domain of the GAL4 transcription factor and RRMs represent the RNA recognition motifs in the HuR protein. ( B ) AH109 yeast cells were (co)transformed with the indicated plasmids. Interactions were tested by growth selection on dropout media lacking the amino acids Leu or Trp (SD/-Leu or SD/-Trp) to select for pGADT7/Rec or <t>pAS2-1</t> plasmid constructs, respectively. Activation of reporter genes was tested on plates lacking Leu, Trp, Hist and Ade, and containing X-α-Gal (SD/-Trp/-Leu/-Hist/-Ade/X-a-Gal).
Gal4 Dna Activation Domain Vector Pact2 (Pas2 1 Tctp, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two+hybrid+y2h+bait+vector+pas2+1/pmc03895383-97-14-21?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
gal4 dna activation domain vector pact2 (pas2-1-tctp - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
human fetal brain cdna library  (Becton Dickinson)
90
Becton Dickinson human fetal brain cdna library
HuR interacts with S-HDAg in a yeast two-hybrid assay . ( A ) Schematic representation of HuR and the AD fusion protein expressed in yeast positive clone (AD/HuR). The protein AD/HuR includes a truncated form of HuR, devoid of the first N-terminal 12 aa. GAL-AD represents the activation domain of the GAL4 transcription factor and RRMs represent the RNA recognition motifs in the HuR protein. ( B ) AH109 yeast cells were (co)transformed with the indicated plasmids. Interactions were tested by growth selection on dropout media lacking the amino acids Leu or Trp (SD/-Leu or SD/-Trp) to select for pGADT7/Rec or <t>pAS2-1</t> plasmid constructs, respectively. Activation of reporter genes was tested on plates lacking Leu, Trp, Hist and Ade, and containing X-α-Gal (SD/-Trp/-Leu/-Hist/-Ade/X-a-Gal).
Human Fetal Brain Cdna Library, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two+hybrid+y2h+bait+vector+pas2+1/pmc03895383-97-31-39?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
human fetal brain cdna library - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


HuR interacts with S-HDAg in a yeast two-hybrid assay . ( A ) Schematic representation of HuR and the AD fusion protein expressed in yeast positive clone (AD/HuR). The protein AD/HuR includes a truncated form of HuR, devoid of the first N-terminal 12 aa. GAL-AD represents the activation domain of the GAL4 transcription factor and RRMs represent the RNA recognition motifs in the HuR protein. ( B ) AH109 yeast cells were (co)transformed with the indicated plasmids. Interactions were tested by growth selection on dropout media lacking the amino acids Leu or Trp (SD/-Leu or SD/-Trp) to select for pGADT7/Rec or pAS2-1 plasmid constructs, respectively. Activation of reporter genes was tested on plates lacking Leu, Trp, Hist and Ade, and containing X-α-Gal (SD/-Trp/-Leu/-Hist/-Ade/X-a-Gal).

Journal: The Open Virology Journal

Article Title: In Vivo Interaction of the Hepatitis Delta Virus Small Antigen with the ELAV-Like Protein HuR

doi: 10.2174/1874357901105010012

Figure Lengend Snippet: HuR interacts with S-HDAg in a yeast two-hybrid assay . ( A ) Schematic representation of HuR and the AD fusion protein expressed in yeast positive clone (AD/HuR). The protein AD/HuR includes a truncated form of HuR, devoid of the first N-terminal 12 aa. GAL-AD represents the activation domain of the GAL4 transcription factor and RRMs represent the RNA recognition motifs in the HuR protein. ( B ) AH109 yeast cells were (co)transformed with the indicated plasmids. Interactions were tested by growth selection on dropout media lacking the amino acids Leu or Trp (SD/-Leu or SD/-Trp) to select for pGADT7/Rec or pAS2-1 plasmid constructs, respectively. Activation of reporter genes was tested on plates lacking Leu, Trp, Hist and Ade, and containing X-α-Gal (SD/-Trp/-Leu/-Hist/-Ade/X-a-Gal).

Article Snippet: The resulting fragment was cleaved ( Mun I/ Bam HI) and inserted into pAS2-1 vector (BD Biosciences) in the local Eco RI/ Bam HI, to obtain the plasmid pAS2-1/S-HDAg.

Techniques: Y2H Assay, Activation Assay, Transformation Assay, Selection, Plasmid Preparation, Construct